Novel pathogen-specific primers for the detection of Agrobacterium vitis and Agrobacterium tumefaciens
نویسندگان
چکیده
1) Dipartimento di Scienze e Tecnologie Agroambientali, Area Patologia Vegetale, Alma Mater Studiorum Universitá di Bologna, Bologna, Italy 2) University of Pécs, Department of Genetics and Molecular Biology, Pécs, Hungary 3) Institut de Biologie Moléculaire des Plantes, Strasbourg, France 4) Cornell University, Department of Plant Pathology, Geneva, USA 5) Research Institute for Viticulture and Enology, Kecskemét, Hungary
منابع مشابه
TEACHING TOOLS IN PLANT BIOLOGYTM: LECTURE NOTES A Really Useful Pathogen, Agrobacterium tumefaciens
Bacteria of the genus Agrobacterium are very useful and unusual plant pathogens. Through a rare interkingdom DNA transfer, the bacteria move some of their genes into their host’s genome, thereby inducing the host cells to proliferate. The result is uncontrolled cell growth leading to a tumor (as induced by Agrobacterium tumefaciens and Agrobacterium vitis) or excessive production of roots (as i...
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Conventional microbiology methods used to detect bacteria include multiple cultures and identification processes, so the results of lab work are painstaking and time-consuming. In recent years, more and more tend to use the diagnostic tests which are based on DNA; hence, DNA diagnostic biosensors have been created to perform DNA identification better. In this study, GUS and hpt genes were used ...
متن کاملMolecular identification of agrobacterium tumefaciens containing pCAMBIA 1305.2 plasmid using multiplex PCR and Gold nanoparticles multiplex probe
Conventional microbiology methods used to detect bacteria include multiple cultures and identification processes, so the results of lab work are painstaking and time-consuming. In recent years, more and more tend to use the diagnostic tests which are based on DNA; hence, DNA diagnostic biosensors have been created to perform DNA identification better. In this study, GUS and hpt genes were used ...
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Rhizobium, Agrobacterium and Allorhizobium are genera within the bacterial family Rhizobiaceae, together with Sinorhizobium. The species of Agrobacterium, Agrobacterium tumefaciens (syn. Agrobacterium radiobacter), Agrobacterium rhizogenes, Agrobacterium rubi and Agrobacterium vitis, together with Allorhizobium undicola, form a monophyletic group with all Rhizobium species, based on comparative...
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Agrobacterium vitis strains, their tumor-inducing (pTi) and tartrate utilization (pTr) plasmid transconjugants and grapevine tumors were analyzed for the presence of N-acyl-homoserine lactones (AHLs). All wild-type A. vitis strains produced long-chain signals. PCR analysis of the A. vitis long-chain AHL synthase gene, avsI, showed the predicted amplicon. Agrobacterium tumefaciens UBAPF2 harbori...
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